Separation of 1,4-benzodiazepine derivates by cyclodextrin modified micellar elektrokinetic capillary chromatography

Written by Hancu Gabriel, Gyéresi Árpád, Gáspár Attila

The aim of this work was to develop a capillary electrophoresis method for the separation of 1,4-benzodiazepine (1,4-BZD) derivates BZD are hydrophobic substances, neutral from electrophoretic point of view, so the most effective method for their separation proved to be micellar elektrokinetic capillary chromatography (MECC). Using a 25 mM sodium tetraborate, 50 mM sodium dodecyl sulphate (SDS) buffer and 7.5% β-CD addition, we managed the baseline separation of the 8 most frequently used BZD derivates. Beside separation we also followed the optimization of the analytical parameters and the method validation. The influence of sodium tetraborate, SDS, β-CD concentrations and the buffer pH upon the separation has been studied. Using the same optimized method but with hydroxy-propyl-β-cyclodextrin, we managed the chiral separation of enantiomers for oxazepam.

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