Plantago lanceolata is spread worldwide, can be found in Europe an Asia, this is a perennial plant. This plant mainly is cultivated, but appears in spontaneous flora, in pastures and in luxuriant grassland. It is used by the ancient times. Therapeutic use dates back to ancient times and is mentioned by medieval ierbarii. The first monograph with this plant product appeared in the Hungarian Pharmacopoeia sixth edition followed by the VIIIth edition as the Plantaginis lanceolatae folium. This paper aims to the pharmacognostical characterization, microscopical study and chemical analysis using the plants leaf. This plant was harvested from the Medicinal Plant Garden UMF Tg. Mures in the year of 2012. Powdered leaves have performed for the microscopical analysis. For phytochemical analysis we used thin layer chromatography to assess the presence of polyphenols and iridoids and a spectrophotometric method for the tannins concentration determination. The thin-layer chromatographic analysis showed the separated flavonides fractions, such as routine, caffeic acid and chlorogenc acid. With TLC we showed the presence of iridoids, such as aucubin. The tannins concentration was 2.98 ± 0.17%. Our results show that the tests used may be extended depending on the possibilities and needs, a method itself does not provide sufficient information on the test substance. After analyzing the leaf of Plantago lanceolata we confirmed the presence of active substances responsible for its beneficial effects in traditional medicine and modern medicine, they can be determined in our laboratory conditions, so it can be investigated by routine methods.
Keywords: Plantaginis lanceolatae folium, active substances: aucubine, flavonoids, tannins